ANTIBIOTIC SUSCEPTIBILITY PROFILE AND MOLECULAR DETECTION OF EXTENDED-SPECTRUM BETA-LACTAMASES IN GRAM-NEGATIVE BACTERIA IN URINE SAMPLES FROM OLABISI ONABANJO UNIVERSITY TEACHING HOSPITAL, SAGAMU, OGUN STATE
Gram-negative bacteria are believed to be important contributors to various forms of urinary tract infections. Therefore, this study aimed to determine the prevalence and resistance pattern of Gram-negative bacteria implicated in urinary tract infection and to detect extended-spectrum beta-lactamase (ESBLs) genes in them. A total of one hundred and twenty (120) clean catch urine samples were collected aseptically from patients attending Olabisi Onabanjo University Teaching Hospital, Sagamu. The urine samples were cultured on Cystine Lactose Electrolyte Deficient (CLED) agar. The isolates were identified using standard biochemical assays. The Kirby-Bauer disk diffusion method was employed to test the antibiotic susceptibility of the isolates. Phenotypic identification and genotypic detection of extended-spectrum beta-lactamase (ESBL) genes in the isolates were performed. Out of the 120 urine samples collected, 55(45.8%) were from females while 65 (54.2%) were from males (<1- >80 years). The prevalence of bacterial isolates in urine samples was 42.5%. The bacterial pathogens isolated were Staphylococcus aureus, Klebsiella pneumoniae, Klebsiella oxytoca, Pseudomonas aeruginosa, Proteus mirabilis, and Escherichia coli. K. pneumoniae was the most common isolate. K. pneumoniae was the most resistant to imipenem (36.8%) and meropenem (42.1%). ESBL genes was found in 24/37 (64.9%). The blaTEM, blaSHV, and blaCTX-M genes were present in 9 (24.3%), 6 (16.2%) and 8 (21.6%) of the isolates, respectively. As a result, increasing public awareness is critical to limiting the spread of antibiotic resistance and necessitates continuous surveillance.
Keywords: Antibiotic resistance, Extended Spectrum Beta-Lactamase, Gram-negative bacteria, Urinary tract infections
*Correspondence: olaniran.oluwatoyin@oouagoiwoye.edu.ng, 09062214760
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